Effects of pulse polarity and pulse delaying time on pulsed electric fields-induced pasteurization of E. coli O157:H7 Original Research Article

Effects of pulse polarity and pulse delaying time on inactivation of E. coli O157:H7 inoculated into apple juice and skim milk samples were determined. Inactivation effect was observed as inoculated food samples were subjected to mono and bipolar square wave pulses. Bipolar pulses with different pulse delaying times were also applied. There was no significant difference between mono (2.56 log10 cfu/ml) and bipolar (2.63 log10 cfu/ml) pulses on the inactivation of E. coli O157:H7 inoculated into apple juice (P > 0.05). However, results in skim milk yielded a significant difference between mono (1.27 log10 cfu/ml) and bipolar (1.96 log10 cfu/ml) pulses with bipolar pulses being significantly more efficient (P < 0.05). Among different pulse delaying times, 20 μs caused a significantly higher inactivation than the others (P < 0.05) in apple juice and skim milk.