Author/Authors :
J.P van Dijken، نويسنده , , J Bauer، نويسنده , , L Brambilla، نويسنده , , P Duboc، نويسنده , , J.M Francois، نويسنده , , C Gancedo، نويسنده , , M.L.F Giuseppin، نويسنده , , J.J. Heijnen، نويسنده , , M Hoare، نويسنده , , H.C. Lange-Jansen، نويسنده , , E.A Madden، نويسنده , , P Niederberger، نويسنده , , J Nielsen، نويسنده , , J.L Parrou، نويسنده , , T Petit، نويسنده , , D Porro، نويسنده , , M Reuss، نويسنده , , N van Riel، نويسنده , , M Rizzi، نويسنده , , H.Y Steensma، نويسنده , , et al.، نويسنده ,
Abstract :
To select a Saccharomyces cerevisiae reference strain amenable to experimental techniques used in (molecular) genetic, physiological and biochemical engineering research, a variety of properties were studied in four diploid, prototrophic laboratory strains. The following parameters were investigated: 1) maximum specific growth rate in shake-flask cultures; 2) biomass yields on glucose during growth on defined media in batch cultures and steady-state chemostat cultures under controlled conditions with respect to pH and dissolved oxygen concentration; 3) the critical specific growth rate above which aerobic fermentation becomes apparent in glucose-limited accelerostat cultures; 4) sporulation and mating efficiency; and 5) transformation efficiency via the lithium-acetate, bicine, and electroporation methods. On the basis of physiological as well as genetic properties, strains from the CEN.PK family were selected as a platform for cell-factory research on the stoichiometry and kinetics of growth and product formation.
Keywords :
Yeast , Physiology , Strain choice , Genetics , Engineering
