Transformation and degradation of the disazo dye Chicago Sky Blue by a purified laccase from Pycnoporus cinnabarinus

The degradation of the disazo dye Chicago Sky Blue 6B by a purified laccase from Pycnoporus cinnabarinus was investigated. Laccase was purified to homogeneity and characterized. The enzyme had a molecular size of 63 kDa as determined by SDS-PAGE and an isoelectric point at pH 3. Amino acid composition and N-terminal amino acid sequence was shown to be similar to other fungal laccases. The purified laccase was stable for 1 h at 60°C and was irreversibly inactivated by sodium azide at 0.1 mM. Laccase was shown to initiate destruction of the chromophore of the disazo dye Chicago Sky Blue, resulting in the formation of two intermediate products with absorption intensities about one order of magnitude lower than the parent molecule. The rate at which the dye was transformed by purified laccase was shown to increase with increasing concentrations of the enzyme.