Title of article
Large-scale production of VHH antibody fragments by Saccharomyces cerevisiae
Author/Authors
Yvonne E. Thomassen، نويسنده , , Wilmar Meijer، نويسنده , , Laurens Sierkstra، نويسنده , , C.Theo Verrips، نويسنده ,
Issue Information
روزنامه با شماره پیاپی سال 2002
Pages
6
From page
273
To page
278
Abstract
We report the large-scale production of VHH antibody fragments, raised against the azo-dye RR-6, by the yeast Saccharomyces cerevisiae. The secretion efficiency of the VHH fragments was correlated with their overall hydrophobicity. With a 15 m3 fed-batch fermentation, 1.3 kg VHHs were produced. Purification was done by removal of biomass and product concentration. During induction, the specific production rate qp was 0.213 g.[kg dryweight]−1.h−1.
Keywords
antibody , VHH , S. cerevisiae , Bulk production , Secretion
Journal title
Enzyme and Microbial Technology
Serial Year
2002
Journal title
Enzyme and Microbial Technology
Record number
1173569
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