The double substrate growth kinetics of Pseudomonas aeruginosa

Growth parameters of Pseudomonas aeruginosa were quantified based on steady-state concentrations, utilization rates of glucose and dissolved oxygen, and microorganism concentration in a chemostat that was operated at 25 °C, pH 7.2, and an agitation rate 350 rpm. The results showed that the microbial growth was limited by the concentration of glucose and the concentration of oxygen. A dual-substrate, Tessier growth kinetics for oxygen and glucose, was in good agreement with the experimental data using the following biokinetic parameters: μmax=0.29 h−1, Kg=26.9 mg/l, Ko=1.18 mg/l, Yx/g=0.628 g microorganism/g glucose and, Yx/o=0.635 g microorganism/g oxygen. Maintenance factors for glucose and oxygen were: mg=0.0078 g glucose consumed/g microorganism h, and mo=0.014 g oxygen consumed/g microorganism h.