Title of article
Purification and characterization of thermo-labile alkaline phosphatase from an Antarctic psychrotolerant Bacillus sp. P9
Author/Authors
Ram Kumar Dhaked، نويسنده , , Syed Imteyaz Alam، نويسنده , , Aparna Dixit، نويسنده , , Lokendra Singh، نويسنده ,
Issue Information
روزنامه با شماره پیاپی سال 2005
Pages
7
From page
855
To page
861
Abstract
A psychrotolerant Bacillus sp. from Antarctica produced an alkaline phosphatase in the culture supernatant. The strain showed 98.4% 16s rDNA sequence identity with Bacillus sphaericus. The 76 kDa protein was purified 11.1-fold showing alkaline phosphomonoesterase activity. Enzyme was optimally produced at 25 °C and pH 7.0. This cold active alkaline phosphatase is heat labile and gets completely inactivated at 60 °C in 50 min and is active in broad pH range.
Keywords
Antarctica , Bacillus sphaericus , Alkaline phosphatase , Psychrotolerant
Journal title
Enzyme and Microbial Technology
Serial Year
2005
Journal title
Enzyme and Microbial Technology
Record number
1174312
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