Title of article :
Activity of magnetite-immobilized catalase in hydrogen peroxide decomposition
Author/Authors :
F. Horst، نويسنده , , E.H. Rueda، نويسنده , , M.L. Ferreira، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2006
Pages :
8
From page :
1005
To page :
1012
Abstract :
The present work analyzes the activity in decomposition of H2O2 using magnetite-immobilized catalase. The support of catalase is a glutaraldehyde-treated magnetite (Fe3O4). The data obtained in the H2O2 decomposition are analyzed. The fitting of the initial rate of the H2O2 decomposition versus hydrogen peroxide concentration data is discussed using a specific program for enzyme kinetics modeling (Leonora). The free catalase from Aspergillus niger (3.5 or 10 U/mL) does not show substrate inactivation up to 0.4 M H2O2. The immobilized catalase at low catalyst concentration shows substrate inhibition. Using 1 mg/mL of supported catalase the predicted maximum activity is higher than in the case of the free catalase at similar catalase concentration, although the optimum temperature is lower (40 °C versus 60 °C).
Keywords :
Hydrogen peroxide , Enzymatic , Catalase , Supported catalase , Magnetite
Journal title :
Enzyme and Microbial Technology
Serial Year :
2006
Journal title :
Enzyme and Microbial Technology
Record number :
1174561
Link To Document :
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