The detection of Salmonella serovars from animal feed and raw chicken using a combined immunomagnetic separation and ELISA method Original Research Article

The immunomagnetic separation technique was used in conjunction with an ELISA-based detection system to recover Salmonella enteritidis, Salmonella typhimurium and Salmonella virchow (2–2×103cfu 25 g−1) from raw chicken and animal feed. The detection time was less than 27 h and included the conventional pre-enrichment (18 h) step in buffered peptone water and an enrichment step (6 h) in glucose nutrient broth. In contrast, the conventional method using Rappaport-Vassiliades enrichment broth and selective plating on XLD agar required 72–96 h.