Interaction of harmine with oligonucleotide d(GTGCAC)2

This work carried out a study on the interaction between harmine and oligonucleotide d(GTGCAC)2. The binding thermodynamic parameters were obtained by ITC method. Harmine binds to oligonuleotide duplex d(GTGCAC)2 in a fashion of intercalation mode and specific interaction and nonspecific interaction exist in the binding process. 1H NMR experiments indicate that oligonucleotide d(GTGCAC)2 is a generally self-complementary B-type duplex molecule, and harmine binds to DNA duplex d(GTGCAC)2 at the side of methoxyl group preferentially in a mode of intercalation.