Author/Authors :
Lito P. de la Rama، نويسنده , , Liang Hu، نويسنده , , Mikhail Y. Efremov، نويسنده , , Eric A. Olson، نويسنده , , Paul F. Nealey، نويسنده , , Mark A. McLean، نويسنده , , Stephen G. Sligar، نويسنده , , Leslie H. Allen، نويسنده ,
Abstract :
Anomalous melting transitions are observed on dimethyl dioctadecyl ammonium bromide (DODAB) using a fast-scanning liquid calorimeter. A single transition is observed at low scan rates (1 K/min) while up to four peaks are evident at high scan rates (60 K/min). These deconvoluted transitions are signatures of the basic properties of the transition kinetic landscape which are convoluted into a single peak at low scanning rates. The technique is also applied to the proteins Rnase A, Lysozyme and Cytochrome P450. The calorimeter is a glass capillary uniformly coated with Ag, which acts both as the heating element and resistive thermometer.
