Synthesis of porous macrospheres from amino-polymers

Porous macrospheres with controlled diameter were produced using N-methylolacrylamide and urea–formaldehyde resins synthesized in our laboratories. Hot silicon oil was used to form the porous macrospheres which were characterized by using infrared spectroscopy (FTIR), scanning electron microscopy, mercury porosimetry and surface area determination (BET). Porous macrospheres of amino-polymers were used as support in immunoassays type ELISA (enzyme linked immunosorbent assay) in which mouse immunoglobulin (IgG) was immobilized, and detected by rabbit anti-mouse immunoglobulin (a-IgG) labeled with horseradish peroxidase. Responses were obtained up to a dilution ratio of 1:4000 of the conjugate standard (1 mg/ml) and 10 μg/ml of IgG solution to recover the spheres in pH=4.5.