Short communication Immunogenicity differences between intact insulin and a peptide representing the C-terminal region

Immunization of rabbits using a peptide corresponding to aminoa cids of the C-terminal region of porcine insulinB –chain produced an an-tipeptide antibody reactive with the native insulin,as well as with the peptide.It was also found that the adsorption characteristics of the anti-peptide antibody against insulin and peptides including its reactivity against the insulin A -chain, were different from those of antinative insulin Antibodies T.he reactivity against the insulin A -chain was raised by maturation of the anti-peptide antibody. These results show that peptide immunization may cause different immuno-responses .and have some advantages for obtaining antibodies possessing desirable characteristics.