Author/Authors :
Dziadczyk، Ewa نويسنده Department of Plant Anatomy and Cytology, Maria Curie-Sk?odowska University Akademicka 19, 20-033 Lublin, Poland , , Domaciuk، Marcin نويسنده Department of Plant Anatomy and Cytology, Maria Curie-Sk?odowska University Akademicka 19, 20-033 Lublin, Poland , , Dziadczyk، Piotr نويسنده Rzesz?w University of Technology, Department of Biochemistry and Biotechnology Aleja Powsta?c?w Warszawy 6, 35-959 Rzesz?w, Poland , , Pawelec ، Iwona نويسنده Department of Plant Anatomy and Cytology, Maria Curie-Sk?odowska University Akademicka 19, 20-033 Lublin, Poland , , Bednara، J?zef نويسنده epartment of Plant Anatomy and Cytology, Maria Curie-Sk?odowska University Akademicka 19, 20-033 Lublin, Poland ,
Abstract :
The purpose of our investigation was to determine appropriate conditions for induction of raspberry (Rubus idaeus cv. Nawojka) cell suspension culture. The established callus culture obtained from leaf explants was used as an inoculum for cell culture initiation. Five combinations of plant growth regulators: 1) 4.0 mg l-1 IAA and 1.0 mg l-1 BAP; 2) 0.25 mg l-1 2,4-D; 3) 0.5 mg l-1 2,4-D; 4) 2.0 mg l-1 NAA and 2.0 mg l-1 BAP; 5) 4.0 mg l-1 NAA and 2.0 mg l-1 BAP, added into modified Murashige and Skoog (1962) medium, were tested in order to get the callus culture suitable for initiation of a cell suspension. The best callus (vigorously growing, healthy and friable) was obtained on the medium supplemented with 4.0 mg l-1 IAA and 1.0 mg l-1 BAP. To find the appropriate culture conditions for dispersing callus tissue in liquid medium into single cells and small aggregates, four combinations of plant hormones (auxins and cytokinins) were tested. The best culture medium for induction of raspberry cv. Nawojka cell suspension appeared to be the one supplemented with 1.0 mg l-1 2,4-D. Also the medium with 8.0 mg l-1 IAA and 1.0 mg l-1 BAP was similarly efficient.
