Adventitious shoot regeneration from dormant buds of persimmon (Diospyros kaki Thunb.) cv. Hachiya

The effects of plant hormones 6-benzylaminopurine (BAP) and indole-3-butyric acid (IBA) on adventitious shoot regeneration from dormant persimmon buds were studied. The object of the study was the persimmon cultivar Hachiya, one of the most important persimmon cultivars in the Mediterranean area and Asia. Shoot regeneration was evaluated 4, 6 and 8 weeks after initiating the hormone treatment. Average shoot length was measured after 8 weeks and was evaluated by LSD test. Except for the media without hormone supplement, there was a statistically significant difference among average values of shoot length of plants, grown on the tested media. The increase in BAP had an effect on shoot regeneration that was significant and more pronounced with the addition of IBA, especially to the MS (1/2 N) medium. The highest value of shoot regeneration (98%) was obtained on medium MS (1/2 N), supplemented with 5 ?mol l–1 BAP and 1 ?mol l–1 IBA, with the highest average shoot length 23.69 mm, measured 8 weeks after the experiment initiation. The results indicate that adventitious shoots can be successfully produced in persimmon cv. Hachiya, especially with the supplement of hormone BAP, which, according to our results, plays an important role in persimmon in vitro regeneration.