Author/Authors :
Kadlec، Jarom?r نويسنده University of South Bohemia, Faculty of Agriculture, ?eské Bud?jovice, Czech Republic , , Hosnedlova، Bo?ena نويسنده University of South Bohemia, Faculty of Agriculture, ?eské Bud?jovice, Czech Republic , , Rehout، V?clav نويسنده University of South Bohemia, Faculty of Agriculture, ?eské Bud?jovice, Czech Republic , , C?tek، Jind?ich نويسنده University of South Bohemia, Faculty of Agriculture, ?eské Bud?jovice, Czech Republic , , Vecerek، Libor نويسنده University of South Bohemia, Faculty of Agriculture, ?eské Bud?jovice, Czech Republic ,
Abstract :
Chicken insulin-like factor 1 gene (IGF1) is a biological candidate gene for the investigation of
growth, body composition, and metabolic and skeletal traits, and is also a positional candidate
gene for growth and fat deposition in chickens. Two broiler populations Ross 308 and Cobb
500 were used to study the relationship between IGF1 gene polymorphism and phenotypic
traits. A single nucleotide polymorphism (SNP) was identified in 132 individuals using the
PCR-RFLP technique. Genotypical frequencies were, for genotype AA: 0.83–0.86, and for AC:
0.14–0.17. Associations between IGF1 promotor polymorphism and liver weight (P?0.05) and
liver weight as a percentage of the weight of the poultry carcass with the giblets (P?0.05),
were found in the AC genotype in a comparison of broiler homozygous chickens AA in the
Cobb 500 line. In these broilers, the breast muscle and leg muscle weight in the AC genotype
were higher, and abdominal fat weight lower compared with AA genotype chickens, but these
differences were not significant.
