FEATURES OF LASER DESORPTION/IONIZATION MASS SPECTROMETRY FRAGMENTATION OF VITAMIN B12

A comparative analysis of the laser desorption/ionization of vitamin B12 by matrix-assisted laser desorption/ionization (MALDI) and desorption/ionization on porous silicon (DIOS) was carried out. The mass spectra obtained were interpreted and the pathways for ion formation and decomposition were established. The MALDI fragmentation of the positive vitamin B12 ions is more extensive than the DIOS fragmentation. The most extensive fragmentation was found using the MALDI method for negative vitamin B12 ions, which are lacking when using the DIOS method.