A Study on th e Effects of IL·10 in Anti-Thy 1-Induced Glomerulonephritis in Rats

In the present study. we examined the effects of IL-10 after 24 h in a model of acute glomerulonephritis (GN). One hour after the anti-Thy I antibody administration. a single i.v. dose of IL- 10 was administered 10 rats. Normnal rats. control nephritic rats and nephritic rats treated with IL- 10. were sacrificed 24 h after administration of antibody. Samples of urine blood and organ, were subsequently collected. The effects of IL•10 were studied by quantification of various inflammatoy parameters at the protein level after immunohistochemical staining and at the mRNA level by a quantitative real time PCR technique , Nitric oxide and protein content were determined in serum and in 24 h-excreced urine. respectively. The inflammatory parameters were reduced in the IL- 10-treated group: in increment in glomerular CD14, ICAM-1 and MMP-13 staining induced by anti-Thy 1 injection was significantly attenuated by IL-10. In contrast. mRNA level, for CD14. IL•-l(beta), TNF•(alpha) and MCP-1 were not different between IL-10-treated and control GN groups. In conclusion. a single i.v. dose of IL-10 suppresses the expression of several inflammatory parameters, 24 h after inducing of acute GN but at this time point mRNA levels of all parameters examined were not affected. Although its thcrapcutic efficacy needs further evaluation. anti -inflammatory effects of this short-lived cytokine can he found one day after its administration.