New methods of proteome analysis: multidimensional chromatography and mass spectrometry

Shortcomings in two-dimensional gel electrophoresis have encouraged the search for new methods of high-throughput proteome analysis. A variety of chromatographic methods can be coupled directly to a mass spectrometer to accomplish this task. However, multidimensional chromatography must be used to achieve the resolving power of two-dimensional gel electrophoresis. Current systems still fall short of the resolving power of two-dimensional gel electrophoresis but they have the potential to improve.