Abstract :
A major challenge in modern biology is the visualization of biochemical pathways in living cells. Subramaniam et al. report on the application of a new technology called ‘protein fragment complementation assays’ (PCAs), which is used to visualize a key step in a signal transduction pathway underlying systemic acquired resistance in higher-plant cells. Their results suggest a wide range of potential applications of their strategy in visualizing biochemical processes and in determining gene function.
Keywords :
2 , plant protoplasts , protein-fragment complementation assays (PCA) , dihydrofolate reductase (DHFR) , TGA2 , systematic acquired resistance (SAR) , 6-dichloroisonicotinic acid (INA) , NPR1/NIM1 , Salicyclic acid (SA)
