Author/Authors :
Gerd Bader، نويسنده , , Susanne Schiffmann، نويسنده , , Anja Herrmann، نويسنده , , Markus Fischer، نويسنده , , Markus Gütlich، نويسنده , , Günter Auerbach، نويسنده , , Tarmo Ploom، نويسنده , , Adelbert Bacher، نويسنده , , Robert Huber، نويسنده , , Thorsten Lemm، نويسنده ,
Abstract :
Tetrahydrobiopterin, the cofactor required for hydroxylation of aromatic amino acids regulates its own synthesis in mammals through feedback inhibition of GTP cyclohydrolase I. This mechanism is mediated by a regulatory subunit called GTP cyclohydrolase I feedback regulatory protein (GFRP). The 2.6 Å resolution crystal structure of rat GFRP shows that the protein forms a pentamer. This indicates a model for the interaction of mammalian GTP cyclohydrolase I with its regulator, GFRP. Kinetic investigations of human GTP cyclohydrolase I in complex with rat and human GFRP showed similar regulatory effects of both GFRP proteins.
Keywords :
tetrahydrobiopterin , regulation , X-ray structure , Biosynthesis , GTP cyclohydrolase I
