Author/Authors :
Rong Zeng، نويسنده , , Rui-Fu Yang، نويسنده , , Mu-De Shi، نويسنده , , Manrong Jiang، نويسنده , , You-Hua Xie، نويسنده , , Hong-Qiang Ruan، نويسنده , , Xiao-Sheng Jiang، نويسنده , , Lv Shi، نويسنده , , Xiao-Hu Zhou PhD، نويسنده , , Lei Zhang، نويسنده , , Xiaodong Wu، نويسنده , , Ying Lin، نويسنده , , Yong-Yong Ji، نويسنده , , Lei Xiong، نويسنده , , Yan Jin، نويسنده , , Er-Hei Dai، نويسنده , , Xiao-Yi Wang، نويسنده , , Bin-Ying Si، نويسنده , , Jin Wang، نويسنده , , Hong-Xia Wang، نويسنده , , Cui-E ، نويسنده ,
Abstract :
Proteomics was used to identify a protein encoded by ORF 3a in a SARS-associated coronavirus (SARS-CoV). Immuno-blotting revealed that interchain disulfide bonds might be formed between this protein and the spike protein. ELISA indicated that sera from SARS patients have significant positive reactions with synthesized peptides derived from the 3a protein. These results are concordant with that of a spike protein-derived peptide. A tendency exists for co-mutation between the 3a protein and the spike protein of SARS-CoV isolates, suggesting that the function of the 3a protein correlates with the spike protein. Taken together, the 3a protein might be tightly correlated to the spike protein in the SARS-CoV functions. The 3a protein may serve as a new clinical marker or drug target for SARS treatment.
Keywords :
3a protein , spike protein , coronavirus , SARS , Proteome
