Eu (III) Indirect Probe to Investigate Interaction of DOPA (3, 4, Di-Hydroxyphenylalanine) and Dopamine (3, 4-Dihydroxy Phenyl Ethyl Amine) Using Electrochemical Methods

Eu (III) can be utilized as an indirect electrochemical probe to investigate the interaction of bio-molecules such as DOPA and Dopamine with Ca (II) in vitro. The interaction of DOPA and Dopamine with Eu(III)-Eu(II) redox pair was investigated by electrochemical techniques. Both the neurotransmitters are structurally related and undergo interaction with Eu (III). Eu (III) coordinates with DOPA and Dopamine through five-member chelate rings via carboxylic oxygen and amino nitrogen. The electrode kinetic parameters viz, transfer coefficient (αna), diffusion coefficient (D), forward heterogeneous rate constant (kofh) were evaluated for Eu(III)-DOPA/Eu(III)-Dopamine. The results obtained were used to investigate the nature of interaction of Eu (III) with multi-donor biomolecules. The possible mechanism was also proposed.