An Intronic Peroxisome Proliferator-activated Receptor-binding sequence Mediates Fatty Acid Induction of the Human Carnitine Palmitoyltransferase 1A

The liver plays a central role in the response to fasting. The hormonal profile in this condition, low insulin, and high concentrations of glucagon in plasma, induce the release of large amounts of fatty acids from adipose tissue. Prolonged starvation can therefore induce a dramatic change in the fatty acid oxidative capacity of liver metabolism. Modulation of gene expression by PPARα plays a crucial role in this response. While a major role for PPARα in the liver is to produce ketone bodies as fuel through β-oxidation for peripheral tissues during fast, its participation in the control of CPT1A, the rate-limiting step of the pathway, remains controversial. Using Web-based software (VISTA) combining transcription factor binding site database searches with comparative sequence analyses, we have localized a conserved functional PPAR responsive element downstream of the transcriptional start site of the human CPT1A gene. We have shown that this sequence is fundamental for fatty acids or PGC1-induced transcriptional activation of the CPT1A gene. These results corroborate the hypothesis that PPARα regulates the limiting step in the oxidation of fatty acids in liver mitochondria.