Title of article
Binding of TmHU to Single dsDNA as Observed by Optical Tweezers
Author/Authors
M. Salomo، نويسنده , , K. Kroy، نويسنده , , K. Kegler، نويسنده , , C. Gutsche، نويسنده , , M. Struhalla، نويسنده , , J. Reinmuth، نويسنده , , W. Skokov، نويسنده , , C. Immisch، نويسنده , , U. Hahn، نويسنده , , R. R. Netz and F. Kremer ، نويسنده ,
Issue Information
روزنامه با شماره پیاپی سال 2006
Pages
8
From page
769
To page
776
Abstract
Optical tweezers are employed to study the action of the histone-like protein from Thermotoga maritima (TmHU) on DNA at a single molecule level. Binding and disruption of TmHU to and from DNA are found to take place in discrete steps of 4–5 nm length and a net binding enthalpy of about 16kBT. This is in reasonable agreement with a microscopic model that estimates the extension of the binding sites of the protein and evaluates the energetics mainly for bending of the DNA in the course of interaction.
Keywords
protein–DNA interaction , optical tweezers , histone-like protein , Thermotoga maritima , single molecule study
Journal title
Journal of Molecular Biology
Serial Year
2006
Journal title
Journal of Molecular Biology
Record number
1248005
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