Title of article :
The Three-dimensional Arcitecture of the EJC Core
Author/Authors :
M. Elizabeth Stroupe، نويسنده , , Thomas ?. Tange، نويسنده , , Dennis R. Thomas، نويسنده , , Melissa J. Moore، نويسنده , , Ludmila Kolmakova-Partensky and Nikolaus Grigorieff، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2006
Pages :
7
From page :
743
To page :
749
Abstract :
The exon junction complex (EJC) is a macromolecular complex deposited at splice junctions on mRNAs as a consequence of splicing. At the core of the EJC are four proteins: eIF4AIII, a member of the DExH/D-box family of NTP-dependent RNA binding proteins, Y14, Magoh, and MLN51. These proteins form a stable heterotetramer that remains bound to the mRNA throughout many different cellular environments. We have determined the three-dimensional (3D) structure of this EJC core using negative-stain random-conical tilt electron microscopy. This structure represents the first structure of a DExH/D-box protein in complex with its binding partners. The EJC core is a four-lobed complex with a central channel and dimensions consistent with its known RNA footprint of about ten nucleotides. Using known X-ray crystallographic structures and a model of three of the four components, we propose a model for complex assembly on RNA and explain how Y14:Magoh may influence eIF4AIIIʹs RNA binding.
Keywords :
spliceosome , exon junction complex , Electron microscopy , three-dimensional structure , MRNA
Journal title :
Journal of Molecular Biology
Serial Year :
2006
Journal title :
Journal of Molecular Biology
Record number :
1248265
Link To Document :
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