Author/Authors :
Dhruti Savalia، نويسنده , , Lars F. Westblade، نويسنده , , Manisha Goel، نويسنده , , Laurence Florens، نويسنده , , Priscilla Kemp، نويسنده , , Natalja Akulenko، نويسنده , , Olga Pavlova، نويسنده , , Julio C. Padovan، نويسنده , , Brian T. Chait and Michael P. Rout، نويسنده , , Michael P. Washburn، نويسنده , , Hans-W. Ackermann، نويسنده , , Arcady Mushegian، نويسنده , , Tarasii Gabisonia، نويسنده , , Ian Molineux، نويسنده , , Konstantin Seve، نويسنده ,
Abstract :
A novel bacteriophage infecting Escherichia coli was isolated during a large-scale screen for bacteriophages that may be used for therapy of mastitis in cattle. The 77,554-bp genome of the bacteriophage, named phiEco32, was sequenced and annotated, and its virions were characterized by electron microscopy and proteomics. Two phiEco32-encoded proteins that interact with host RNA polymerase were identified. One of them is an ECF family σ factor that may be responsible for transcription of some viral genes. Another RNA polymerase-binding protein is a novel transcription inhibitor whose mechanism of action remains to be defined.
Keywords :
Podoviridae , E. coli , MudPIT , GENOME , Bacteriophage
