Author/Authors :
E. Milhiet، نويسنده , , D. COMAS، نويسنده , , A. Richard، نويسنده , , G. Didelot، نويسنده , , A. Débarre، نويسنده , , T. Preat، نويسنده , , P. Tchenio، نويسنده ,
Abstract :
Fluorescence in situ hybridization (ISH) could be a powerful tool for analyzing the dynamics of memory phases if a semi-quantitative measurement of mRNA levels could be performed with a cellular resolution. While such a method does not yet exist, we show preliminary results in that direction where direct labeling of RNA probes with fluorescent dyes is combined with single-molecule-like detection.
Keywords :
in situ hybridization , Wide-field fluorescence microscopy , Memory genes , Single Molecule , Quantitative imaging , Drosophila
