The application of porous ZnO 3D framework to assemble enzyme for rapid and ultrahigh sensitive biosensors

A porous 3 dimensional (D) ZnO framework was constructed by electrospinning. The framework was consisted of connected mesoporous nanofibers and used as excellent matrix to assemble horseradish peroxidase. A novel enzymatic biosensor based on the mesoporous ZnO nanofibers/chitosan inorganic–organic composite 3D spatial framework was prepared. The as-fabricated biosensor exhibited ultrahigh sensitivity (1910.1 μA cm−2 mM−1), fast response (<3 s), low detection limit (1 μ), good reproducibility and stability. The excellent performance is attributed to the 1D ZnO nanofibers with mesopores for enzyme loading, connected 3D integrated framework for excellent electrical transport properties and good biocompatibility of chitosan. The 3D porous ZnO framework provided an ideal environment for enzyme assembly.