A search for pyrophosphate mimics for the development of substrates and inhibitors of glycosyltransferases Original Research Article

The design and synthesis of several β-1,4-galactosyltransferase inhibitors are reported. Mimics of the pyrophosphate-Mn2+ complex were the focus of the design. Malonic, tartaric, and monosaccharide moieties were used as replacements of the pyrophosphate moiety, and galactose or azasugars with potent galactosidase inhibitory activity were used as the ʹdonorʹ component. Compound 6, in which glucose was used as the pyrophosphate-Mn2+ complex mimic and galactose as the ʹdonorʹ component, showed the best inhibitory activity towards the transferase with a K??? of 119.6 μM.