Chemoenzymatic synthesis of l-galactosylated dimeric Sialyl Lewis X structures employing α-1,3-fucosyltransferase V Original Research Article

l-Galactosylated dimeric sialyl Lewis X (SLex) has been prepared employing a combination of chemical and enzymatic synthetic methods. GDP-l-galactose has been chemically synthesised. Enzymatic transfer of l-galactose onto the acceptor (Sia-α2,3-Gal-β1,4-GlcNAc-β1,3/6)2-Man-α1-OMe was achieved using the human α-1,3-fucosyltransferase V.