Control of reaction chemoselectivity with a circular DNA template in the ligation of short oligodeoxyribonucleotides Original Research Article

A study has been conducted to examine the chemoselective control attained in the ligation of short oligodeoxyribonucleotides (ODNs) directed by association on a circular DNA template through triplex formation relative to the same ligation on a single-strand DNA template through duplex formation. The highly efficient, chemospecific ligation achieved under a variety of conditions on the circular DNA template presumably arises from the higher substrate–template association resulting in improved transition state accessibility and protection of the ODNs from side reactions.