Author/Authors :
Wendelin Frick، نويسنده , , Andrea Bauer-Sch?fer، نويسنده , , Jochen Bauer، نويسنده , , Frank Girbig، نويسنده , , Daniel Corsiero، نويسنده , , Hubert Heuer، نويسنده , , Werner Kramer، نويسنده ,
Abstract :
The design and synthesis of a biotin-tagged photoreactive analogue C-4 of the cholesterol absorption inhibitor Ezetimibe is described. Photoaffinity labeling of intestinal brush border membrane vesicles with C-4 and subsequent streptavidin–biotin chromatography leads to selective extraction of a 145 kDa integral membrane protein as the molecular target for cholesterol absorption inhibitors.
