Title of article :
Two novel antibiotics, Sch 419558 and Sch 419559, produced by Pseudomonas fluorescens: effect on activity by overexpression of RpoE Original Research Article
Author/Authors :
Shu-Wei Yang، نويسنده , , Ling Xu، نويسنده , , Ronald Mierzwa، نويسنده , , Ling He، نويسنده , , Joseph Terracciano، نويسنده , , Mahesh Patel، نويسنده , , Vincent Gullo، نويسنده , , Todd Black، نويسنده , , Wenjun Zhao، نويسنده , , Tze-Ming Chan، نويسنده , , Min Chu، نويسنده ,
Abstract :
Two new secondary metabolites designated as Sch 419558 (1) and Sch 419559 (2), were isolated from the fermentation broth of Pseudomonas fluorescens. Structure elucidation of 1 and 2 was accomplished by spectroscopic data analyses including MS and NMR experiments. Both compounds were identified as lipopeptides containing valine and threonine linked with 1-amino-1-hydroxy-heptadec-9-en-2-one or 1-amino-1-hydroxy-pentadecan-2-one carbon chains, respectively. Characterization of the amino acids was further confirmed by amino acid analysis. Compounds 1 and 2 exhibited antibacterial activity against a sensitized E. coli strain with minimum inhibitory concentration of 0.3 and 0.6 μg/mL, respectively. Overexpression of RpoE in the E. coli strain increased the MIC over 60-fold for compounds 1 and 2.
