Title of article
Sequence-specific Methyltransferase-Induced Labelling (SMILing) of plasmid DNA for studying cell transfection Original Research Article
Author/Authors
Falk H.-G. Schmidt، نويسنده , , Michael Hüben، نويسنده , , Basar Gider، نويسنده , , François Renault، نويسنده , , Marie-Paule Teulade-Fichou، نويسنده , , Elmar Weinhold، نويسنده ,
Issue Information
روزنامه با شماره پیاپی سال 2008
Pages
9
From page
40
To page
48
Abstract
Plasmid DNA (pUC19 and pBR322) was sequence-specifically, covalently labelled with Cy3 fluorophores using a newly synthesised N-adenosylaziridine cofactor and the DNA methyltransferase M.TaqI. The fluorescently labelled plasmids were used for transfection of mammalian cells and their intracellular distribution was visualised by epifluorescence and confocal fluorescence microscopy. Although these prokaryotic plasmids do not contain nuclear import sequences, translocation into the nuclei was observed.
Keywords
Cofactor engineering , Aziridine cofactor , Enzymatic DNA labelling , DNA methyltransferase , DNA modification , DNA trafficking , Cell transfection
Journal title
Bioorganic and Medicinal Chemistry
Serial Year
2008
Journal title
Bioorganic and Medicinal Chemistry
Record number
1303876
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