High-mannose-type glycan modifications of dihydrofolate reductase using glycan–methotrexate conjugates Original Research Article

Various high-mannose-type glycan modifications of dihydrofolate reductase (DHFR) were achieved by ligand-based approach using glycan–methotrexate (MTX) conjugates as tight binding glycan bearing ligands for DHFR. The resulting glycan–MTX conjugates and the corresponding artificial glycoproteins could be useful as oligosaccharide- and glycoprotein-probes to perform quantitative analysis of glycan recognizing protein such as lectins, glycosyltransferases or glycosidases. Moreover, artificial glycoproteins having two different high-mannose-type glycans were developed for the first time by a combination of two different types of glycan modification strategies.