Practical syntheses of dyes for difference gel electrophoresis Original Research Article

The three dyes (two indocyanine and one benzoxazolium) useful in difference gel electrophoresis—methyl Cy5 1, propyl Cy3 2, and the benzoxazolium dye Cy2 3—and their NHS esters have been prepared by efficient routes in good overall yield from inexpensive precursors.