Title of article
Effects of ascorbic acid on sperm motility, viability, acrosome reaction and DNA integrity in teratozoospermic samples
Author/Authors
Fanaei، Hamed نويسنده Pregnancy Health Research Center, Zahedan University of Medical Sciences, Zahedan, Iran. , , Khayat، Samira نويسنده Pregnancy Health Research Center, Zahedan University of Medical Sciences, Zahedan, Iran. , , Halvaei، Iman نويسنده Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran , , Ramezani، Vahid نويسنده Department of Pharmaceutics, Faculty of Pharmacy, Shahid Sadoughi University of Medical Sciences, Yazd, Iran , , Azizi، Yaser نويسنده Department of Physiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran , , Kasaeian، Amir نويسنده Non-Communicable Diseases Research Center, Endocrinology and Metabolism Population Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran. , , Mardaneh، Jalal نويسنده Prof. Alborzi Clinical Microbiology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran , , Parvizi، Mohammad-Reza نويسنده , , Akrami، Maryam نويسنده Kashan University of Medical Sciences, Kashan, Iran ,
Issue Information
ماهنامه با شماره پیاپی سال 2014
Pages
8
From page
103
To page
110
Abstract
Background: Oxidative stress in teratozoospermic semen samples caused poor assisted reproductive techniques (ART) outcomes. Among antioxidants, ascorbic acid is a naturally occurring free radical scavenger and as such its presence assists various other mechanisms in decreasing numerous disruptive free radical processes. Objective: The main goal of this study was to evaluate potential protective effects of ascorbic acid supplementation during in vitro culture of teratozoospermic specimens. Materials and Methods: Teratozoospermic semen samples that collected from 15 volunteers were processed, centrifuged and incubated at 37oC until sperm swimmed-up. Supernatant was divided into four groups and incubated at 37oC for one hour under different experimental conditions: Control, 10 ?m A23187, 600?m ascorbic acid and 10 ?m A23187+600 ?m ascorbic acid. After incubation sperm motility, viability, acrosome reaction, DNA damage and malondialdehyde levels were evaluated. Results: Our results indicated that after one hour incubation, ascorbic acid significantly reduced malondialdehyde level in ascorbic acid group (1.4±0.11 nmol/ml) compared to control group (1.58±0.13 nmol/ml) (p < 0.001). At the end of incubation, progressive motility and viability in ascorbic acid group (64.5±8.8% and 80.3±6.4%, respectively) were significantly (p < 0.05 and p < 0.001, respectively) higher than the control group (54.5±6.8% and 70.9±7.3%, respectively). A23187 significantly (p < 0.0001) increased acrosome reaction in A23187 group (37.3±5.6%) compared to control group (8.5±3.2%) and this effect of A23187 attenuated by ascorbic acid in ascorbic acid+A23187 group (17.2±4.4%). DNA fragmentation in ascorbic acid group (20±4.1%) was significantly (p < 0.001) lower than controls (28.9±4.6%). Conclusion: In vitro ascorbic acid supplementation during teratozoospermic semen processing for ART could protect teratozoospermic specimens against oxidative
stress, and it could improve ART outcome.
Journal title
International Journal of Reproductive BioMedicine
Serial Year
2014
Journal title
International Journal of Reproductive BioMedicine
Record number
1314259
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