Author/Authors :
Fanaei، Hamed نويسنده Pregnancy Health Research Center, Zahedan University of Medical Sciences, Zahedan, Iran. , , Khayat، Samira نويسنده Pregnancy Health Research Center, Zahedan University of Medical Sciences, Zahedan, Iran. , , Halvaei، Iman نويسنده Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran , , Ramezani، Vahid نويسنده Department of Pharmaceutics, Faculty of Pharmacy, Shahid Sadoughi University of Medical Sciences, Yazd, Iran , , Azizi، Yaser نويسنده Department of Physiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran , , Kasaeian، Amir نويسنده Non-Communicable Diseases Research Center, Endocrinology and Metabolism Population Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran. , , Mardaneh، Jalal نويسنده Prof. Alborzi Clinical Microbiology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran , , Parvizi، Mohammad-Reza نويسنده , , Akrami، Maryam نويسنده Kashan University of Medical Sciences, Kashan, Iran ,
Abstract :
Background: Oxidative stress in teratozoospermic semen samples caused poor assisted reproductive techniques (ART) outcomes. Among antioxidants, ascorbic acid is a naturally occurring free radical scavenger and as such its presence assists various other mechanisms in decreasing numerous disruptive free radical processes. Objective: The main goal of this study was to evaluate potential protective effects of ascorbic acid supplementation during in vitro culture of teratozoospermic specimens. Materials and Methods: Teratozoospermic semen samples that collected from 15 volunteers were processed, centrifuged and incubated at 37oC until sperm swimmed-up. Supernatant was divided into four groups and incubated at 37oC for one hour under different experimental conditions: Control, 10 ?m A23187, 600?m ascorbic acid and 10 ?m A23187+600 ?m ascorbic acid. After incubation sperm motility, viability, acrosome reaction, DNA damage and malondialdehyde levels were evaluated. Results: Our results indicated that after one hour incubation, ascorbic acid significantly reduced malondialdehyde level in ascorbic acid group (1.4±0.11 nmol/ml) compared to control group (1.58±0.13 nmol/ml) (p < 0.001). At the end of incubation, progressive motility and viability in ascorbic acid group (64.5±8.8% and 80.3±6.4%, respectively) were significantly (p < 0.05 and p < 0.001, respectively) higher than the control group (54.5±6.8% and 70.9±7.3%, respectively). A23187 significantly (p < 0.0001) increased acrosome reaction in A23187 group (37.3±5.6%) compared to control group (8.5±3.2%) and this effect of A23187 attenuated by ascorbic acid in ascorbic acid+A23187 group (17.2±4.4%). DNA fragmentation in ascorbic acid group (20±4.1%) was significantly (p < 0.001) lower than controls (28.9±4.6%). Conclusion: In vitro ascorbic acid supplementation during teratozoospermic semen processing for ART could protect teratozoospermic specimens against oxidative
stress, and it could improve ART outcome.
