Author/Authors :
Rahbarian، N نويسنده Cellular and Molecular Biology Research Center, Shahid Behesthi University, M.C., Tehran, Iran , , Mesgarian ، A نويسنده Dept. of Medical Parasitology and Mycology, Medicine Faculty, Shahid Behesthi University, M.C., Tehran, Iran , , Mahmoudi، Rad M نويسنده Cellular and Molecular Biology Research Center, Shahid Behesthi University, M.C., Tehran, Iran , , Hajaran ، H نويسنده Dept. of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran , , Shahbazi ، F نويسنده Dept. of Medical Parasitology and Mycology, Medicine Faculty, Shahid Behesthi University, M.C., Tehran, Iran , , Mesgarian، Z نويسنده Gonbad-e Qabus Health Center, Golestan, Iran , , Taghipour ، N نويسنده Dept. of Medical Parasitology and Mycology, Medicine Faculty, Shahid Behesthi University, M.C., Tehran, Iran ,
Abstract :
Abstract
Background: To determine the epidemiological status of cutaneous leishmaniasis outbreak, isolation and identification of the agent parasite, Leishmania, using PCR method in Gonbad-e Qabus County, north Iran, during 2006-2007.
Methods: Data were collected on the prevalence of scars and ulcers over a period of 3 months among 6990 inhabitants of five villages around Gonbad-e Qabus County, north Iran, during 2006-2007. Cul-tured promastigotes were identified using PCR technique. Its1 and its2 of Non Coding Transcribed re-gion at ribosomal DNA of 46 Leishmania isolates were amplified and the PCR products were sepa-rated by electrophoresis in 1.5% agarose gel (200 mA, 140 V), visualized by staining with ethidium bro mide, and photographed.
Results: Among 6990 inhabitants of 5 villages, 62.9% were identified as scars and 1.5% as active le-sions. Individuals 11 to 20 years were the most highly infected age group. All the parasite isolates were Leishmania major.
Conclusion: Cutaneous leishmaniasis due to L. major is endemic in Gonbad-e Qabus County, north Iran.
