Author/Authors :
Maghsood، Amir Hossein نويسنده Department of Medical Parasitology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran , , Jadideslam، Golamreza نويسنده Department of Medical Parasitology and Mycology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran , , Fallah، Mohammad نويسنده , , Bazmani، Ahad نويسنده Research Center of Infectious Diseases and Tropical Medicine, Tabriz University of Medical Science, Tabriz, Iran . ,
Abstract :
Background: Leishmaniasis is a tropical disease that is endemic in some areas of Iran, including
East Azerbaijan. IFN-γ is one of the cytokines that triggers cell-mediated immunity, thus initiating
elimination of the infection. This case-control study was performed to investigate the association
between the polymorphism of the IFN-γ gene at the +874A/T locus and visceral leishmaniasis
(VL).
Methods: In this study conducted during 2012-2013, 267 participants were selected from individuals
living in an endemic area of VL. Subjects were divided into three groups; 86 patients with
VL, 82 seropositive individuals without any history of leishmaniasis, and 99 seronegative healthy
controls. Genotyping of the IFN-γ +874A/T polymorphism was carried out using an Amplification
Refractory Mutation System-PCR (ARMS-PCR).
Results: The frequency of the +874A allele in the patient group (75.5%) was higher than in the
seropositive individuals (54%). The highest frequency of the +874T/T genotype was observed in
seropositive individuals, while the patient group had the lowest frequency (34.1% vs. 24.5%).
However, these differences were not significant.
Conclusions: There was no significant association between IFN-γ +874A/T polymorphism and
VL.
