Determination of Folic Acid in Mint Vegetable, by High-Performance Liquid Chromatography

We have applied a quick and convenient method for determining folic acid (FA) excreted in mint vegetable. Sample-treatment entails SPE with Sep-Pak C18 (0.9 g) cartridge. The water-soluble vitamin were analyzed by HPLC on a Discovery C-18 150mm×4.6mm column with 0.1 mol.L-1 KH2PO4 (pH 7.0), methanol, 90:10, as mobile phase (0.7 mL min-1) in isocratic mode.