Title of article
Improved protocol for isolation of genomic DNA from leaf tissues of Phyllanthus emblica Gaertn
Author/Authors
Nagarajan، Sangeetha نويسنده Department of Biotechnology, Plant Genetic Improvement Laboratory, Sri Paramakalyani Centre for Environmental Sciences, Manonmaniam Sundaranar University, Alwarkurichi 627 412, Tirunelveli District, Tamilnadu, India , , Steephen، Mercy نويسنده Department of Biotechnology, Plant Genetic Improvement Laboratory, Sri Paramakalyani Centre for Environmental Sciences, Manonmaniam Sundaranar University, Alwarkurichi 627 412, Tirunelveli District, Tamilnadu, India , , Murugan، Kavitha نويسنده Department of Biotechnology, Plant Genetic Improvement Laboratory, Sri Paramakalyani Centre for Environmental Sciences, Manonmaniam Sundaranar University, Alwarkurichi 627 412, Tirunelveli District, Tamilnadu, India , , Raveendran Nair، Rahul نويسنده Department of Biotechnology, Plant Genetic Improvement Laboratory, Sri Paramakalyani Centre for Environmental Sciences, Manonmaniam Sundaranar University, Alwarkurichi 627 412, Tirunelveli District, Tamilnadu, India , , Sethuraman، Thilaga نويسنده Department of Biotechnology, Plant Genetic Improvement Laboratory, Sri Paramakalyani Centre for Environmental Sciences, Manonmaniam Sundaranar University, Alwarkurichi 627 412, Tirunelveli District, Tamilnadu, India , , Alagar، Parameswari نويسنده Department of Biotechnology, Plant Genetic Improvement Laboratory, Sri Paramakalyani Centre for Environmental Sciences, Manonmaniam Sundaranar University, Alwarkurichi 627 412, Tirunelveli District, Tamilnadu, India , , Ganesh، Doss نويسنده Department of Plant Biotechnology, School of Biotechnology, Madurai Kamarai University, Palkalainagar, Madurai 625 021, India ,
Issue Information
فصلنامه با شماره پیاپی 0 سال 2011
Pages
7
From page
307
To page
313
Abstract
Modified Cetyltrimethylammonium bromide (CTAB) protocol for DNA isolation was developed from leaf tissues of Phyllanthus emblica for obtaining high quality genomic DNA. Fresh leaves of three different maturity were analyzed for yield and quality of DNA. Acidity was determined in three different maturity of leaves viz. tender, intermediate and mature and their influence on DNA quality was determined. Drastic reduction of pH was the primary cause for poor quality of DNA. However, high quality DNA isolation was achieved by stabilizing the pH by addition of NaOH during different stages of DNA isolation process. The present protocol yielding high quality intact DNA for genetic fingerprinting as well as for amplification of chloroplast genes for molecular analysis.
Journal title
Iranian Journal of Biotechnology (IJB)
Serial Year
2011
Journal title
Iranian Journal of Biotechnology (IJB)
Record number
1314562
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