Author/Authors :
Doan، Chinh Chung نويسنده Faculty of Biology, University of Science, Vietnam National University, Ho Chi Minh city, Vietnam Doan, Chinh Chung , Le، Thanh Long نويسنده Dept. of Animal Biotechnology, Institute of Tropical Biology, Vietnam Academy of Science and Technology, Ho Chi Minh City, Vietnam Le, Thanh Long , Hoang، Nghia Son نويسنده Dept. of Animal Biotechnology, Institute of Tropical Biology, Vietnam Academy of Science and Technology, Ho Chi Minh City, Vietnam Hoang, Nghia Son , Doan، Ngoc Trung نويسنده Faculty of Biology, University of Science, Vietnam National University, Ho Chi Minh city, Vietnam Doan, Ngoc Trung , Le، Van Dong نويسنده Dept. of Immunology, Vietnam Military Medical University, Hanoi, Vietnam Le, Van Dong , Do، Minh Si نويسنده Faculty of Biology, University of Science, Vietnam National University, Ho Chi Minh city, Vietnam Do, Minh Si
Abstract :
Background: Stem cell therapy for the treatment of vascular-related diseases through functional revascularization is one of the most important research areas in tissue engineering. The aim of this study was to investigate the in vitro differentiation of umbilical CL-MSC into endothelial lineage cells. Methods: In this study, isolated cells were characterized for expression of MSC-specific markers and osteogenic and adipogenic differentiation. They were induced to differentiate into endothelial-like cells and then examined for expression of the endothelial-specific markers, karyotype, and functional behavior of cells. Results: Isolated cells expressed MSC-specific markers and differentiated into adipocytes and osteoblasts. After endothelial differentiation, they expressed CD31, vWF, VE-cadherin, VEGFR1, and VEGFR2 at both mRNA and protein level, but their morphological changes were not apparent when compared with those of undifferentiated cells. There were no significant changes in karyotype of differentiated cells. Furthermore, angiogenesis assay and LDL uptake assay showed that differentiated cells were able to form the capillary-like structures and uptake LDL, respectively. Conclusion: The results indicated that umbilical CL-MSC could differentiate into functional endothelial-like cells. Also, they are suitable for basic and clinical studies to cure several vascular-related diseases. Iran. Biomed. J. 18 (2): 67-75, 2014
