Author/Authors :
Ghasempour، Hamidreza نويسنده Department of Biotechnology and Chemical Engineering, Science and Research branch, Islamic Azad University, Kermanshah, Iran , , Soheilikhah، Zhaleh نويسنده Department of Biology, Razi University, Kermanshah, Iran , , Zebarjadi، Ali Reza نويسنده Department of Plant Breeding and Agronomy, Faculty of Agriculture, Razi University, Kermanshah, Iran , , Ghasempour، Saba نويسنده School of Biological Sciences and Biotechnology, Faculty of Sciences and Engineering, Murdoch University, Perth, Western Australia , , Karimi، Naser نويسنده Department of Biology, Faculty of Science, Razi University, Kermanshah, I. R. Iran ,
Abstract :
The present study was conducted to induce callus from different in vitro grown seedling explants of shoot, root and leaf segments in order to develop shoot buds and rooting from callus. The sterilized seeds were cultured on Murashige and Skoog medium, and then the explants were cultured from seedling and transferred to an MS medium supplemented with different concentrations of BAP, kinetin, NAA, and 2, 4-D growth regulator hormones. Among all explants, the leaf explants should produce significant callus induction (97.79 %) on MS + 1.0 mgl-1 BAP medium. After 4 weeks, the obtained calluses were transferred to a new regeneration media with different concentrations of BAP and NAA and then the calluses were sub cultured on the same medium every 2 weeks. Overall, the maximum regeneration frequency was obtained from MS medium containing 0.1 mgl-1 NAA and 1.0 mgl-1 BAP.
