Author/Authors :
Rafieian-kopaei، Mahmoud نويسنده Department of Pharmacology, Medical Plants Research Center, Shahr-e-kord University of Medical Sciences, Shahr-e-Kord, Iran Rafieian-kopaei, Mahmoud , Bahmani ، Mahmoud نويسنده 1Urmia University of Medical Sciences, Urmia, Iran , , Eftekhari، Zohre نويسنده 3Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran , , Banihabib، Ebrahimkhalil نويسنده 4Tabriz University of Medical Sciences, Tabriz, Iran , , Hajigholizadeh، Ghader نويسنده 1Urmia University of Medical Sciences, Urmia, Iran , , Bahmani، Farzaneh نويسنده 5Department of Entomology, Ahvaz Jondishapour University of Medical Sciences, Ahvaz, Iran , , Azadzadeh، Jafar نويسنده 1Urmia University of Medical Sciences, Urmia, Iran , , Abdollahi، Mohammad Reza نويسنده , , Kheyri، Aghakhan نويسنده 1Urmia University of Medical Sciences, Urmia, Iran , , Sotoudeh، Abdolsalam نويسنده 1Urmia University of Medical Sciences, Urmia, Iran , , Karamati، Seyed Ahmad نويسنده 1Urmia University of Medical Sciences, Urmia, Iran , , Jelodari، Mahyar نويسنده 6Urmia University, Urmia, Iran ,
Abstract :
Leeches had several complications such as pain, itching, inflammation, severe anemia, short-term bleeding, hypersensitivity, and anaphylactic reactions in their hosts. Harmal Peganum harmala L. is used as an analgesic and anti-inflammatory agent and it has antibacterial activity. Olive Olea europaea L. has antibacterial, anti-viral, hypoglycemic and the relaxation of blood vessels properties. Antioxidant properties of olive also had been reported. This study was carried out to detect the effects of methanolic extracts of P. harmala L. and O. europaea L. on L. nilotica immature form. In 2011, 55 immature leeches collected from the southern area of Ilam province were prepared. The methanolic extract of O. europaea L and P. harmala L. were compared with levamisole as the control drug. Distilled water was evaluated as the placebo group which investigated L. nilotica using anti-leech assay. Then extract and drugs were added and their effects were screened for 720 min and time to paralyze, kill and death of each leech was recorded. The results showed that olive methanolic extractions (600 and 300mg) could kill the leeches in an average time of 145±77.57 and171±33.28 min, respectively. An average death time for levamisole was found to be 15±7.49 min. The highest effectiveness was found for levamisole at dose 300 mg. Methanol extracts of the Harmal (300 and 600 ?g/m) and springs water showed no anti-leech. In sum, olive plant could use for anti Limnatis nilotica expenditure.
