Author/Authors :
Asadi-Shekaari، Majid نويسنده Electron Microscopy Department, Kerman Neuroscience Research Center (KNRC), Kerman, Iran , , Eftekhar Vaghefi، Seyed Hassan نويسنده , , Abadi pour، Masoud نويسنده Dept. of Anatomy, School of Medicine, Kerman University of Medical Sciences, Kerman , , Sheibani، Vahid نويسنده Neuroscience Research Center, Kerman University of Medical Sciences, Kerman , , Shams Ara، Ali نويسنده Dept. of Anatomy, School of Medicine, Kerman University of Medical Sciences, Kerman , , Behbahani، Parisima نويسنده Central Lab, Shahid Chamran University of Medical Sciences, Ahwaz ,
Abstract :
Background and Objective: As one of the widely used drugs, aspirin (acetyl-salicylic acid, ASA)
plays an important role in stroke treatment and prevention. In a previous study, we demonstrated
ASA injection at 30 min after ischemia onset is neuroprotective. To determine whether the neurons
protected by ASA had a normal ultrastructure, hippocampal CA1 pyramidal neurons were examined
by Transmission Electron Microscope (TEM).
Material and Methods: Adult male wistar rats were divided into three different groups (6 animals/
group): Sham-operated, control (48 MCAO+vehicle) and aspirin (48 MCAO + ASA). ASA (30 mg/kg) was injected 30 min after ischemia onset. The animals were killed 2 days after ischemia induction and their brain removed, processed, and examined under a TEM.
Results: Apoptotic changes were observed in rats not treated with ASA. In contrast, pyramidal neuron ultrastructure appeared normal in rats that exhibited neuroprotection (defined at the light microscope level) by ASA when studied two days after ischemia.
Conclusion: We conclude that administration of ASA after permanent focal cerebral ischemia
remains a considerable therapeutic strategy.
