Author/Authors :
Sumitomo، نويسنده , , Makoto and Iwase، نويسنده , , Akira and Zheng، نويسنده , , Rong and Navarro، نويسنده , , Daniel and Kaminetzky، نويسنده , , David and Shen، نويسنده , , Ruoqian and Georgescu، نويسنده , , Maria-Magdalena and Nanus، نويسنده , , David M، نويسنده ,
Abstract :
We show in this study that endogenous NEP and PTEN associate in cells directly through electrostatic interactions between a highly basic residue stretch in the intracellular domain of NEP and the major phosphorylation site in PTENʹs tail. NEP binds and engages in higher order complexes both phosphorylated and unphosphorylated PTEN. NEP recruits PTEN to the plasma membrane and enhances its stability and phosphatase activity. As a result, an enzymatically inactive NEP mutant preserves the ability to bind PTEN, inactivates the Akt/PKB kinase, and partially suppresses the growth of PC cells. This study demonstrates a molecular cooperation between NEP and PTEN tumor suppressors in which NEP constitutively recruits and activates PTEN to inhibit the PI3K/Akt oncogenic pathway.
