Title of article :
Thymidylate synthase as an oncogene: A novel role for an essential DNA synthesis enzyme
Author/Authors :
Rahman، نويسنده , , Lambratu and Voeller، نويسنده , , Donna and Rahman، نويسنده , , Monzur and Lipkowitz، نويسنده , , Stan and Allegra، نويسنده , , Carmen and Barrett، نويسنده , , J.Carl and Kaye، نويسنده , , Frederic J and Zajac-Kaye، نويسنده , , Maria، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2004
Pages :
11
From page :
341
To page :
351
Abstract :
Thymidylate synthase (TS) is an E2F1-regulated enzyme that is essential for DNA synthesis and repair. TS protein and mRNA levels are elevated in many human cancers, and high TS levels have been correlated with poor prognosis in patients with colorectal, breast, cervical, bladder, kidney, and non-small cell lung cancers. In this study, we show that ectopic expression of catalytically active TS is sufficient to induce a transformed phenotype in mammalian cells as manifested by foci formation, anchorage independent growth, and tumor formation in nude mice. In contrast, comparable levels of two TS mutants carrying single point mutations within the catalytic domain had no transforming activity. In addition, we show that overexpression of TS results in apoptotic cell death following serum removal. These data demonstrate that TS exhibits oncogene-like activity and suggest a link between TS-regulated DNA synthesis and the induction of a neoplastic phenotype.
Journal title :
Cancer Cell
Serial Year :
2004
Journal title :
Cancer Cell
Record number :
1335399
Link To Document :
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