Author/Authors :
Ohira، نويسنده , , Miki and Oba، نويسنده , , Shigeyuki and Nakamura، نويسنده , , Yohko and Isogai، نويسنده , , Eriko and Kaneko، نويسنده , , Setsuko and Nakagawa، نويسنده , , Atsuko and Hirata، نويسنده , , Takahiro and Kubo، نويسنده , , Hiroyuki and Goto، نويسنده , , Takeshi and Yamada، نويسنده , , Saichi and Yoshida، نويسنده , , Yasuko and Fuchioka، نويسنده , , Misa and Ishii، نويسنده , , Shin and Nakagawara، نويسنده , , Akira، نويسنده ,
Abstract :
Summary
dict the prognosis of neuroblastoma patients and choose a better therapeutic protocol, we developed a cDNA microarray carrying 5340 genes obtained from primary neuroblastomas and examined 136 tumor samples. We made a probabilistic output statistical classifier that provided a high accuracy in prognosis prediction (89% at 5 years) and a highly reliable method to validate it. Kaplan-Meier analysis indicated that the patients in an intermediate group defined by existing markers are divided by microarray into two further groups with 5 year survivals for 36% and 89% of patients (p < 10−4), i.e., with unfavorably and favorably predicted neuroblastomas, respectively. According to these results, we developed a gene subset chip for a clinical tool, for which our classifier exhibited 88% prediction accuracy.
