Author/Authors :
Javad، Baharara نويسنده Associate Professor, Animal Developmental Biology, Research Center for Animal Development Applied Biology& Dept. Biology, Islamic Azad University, Mashhad, Iran , , Tayebe، Ramezani نويسنده PhD Student in Developmental Biology, Department of Animal Biology, Kharazmi University, Tehran, Iran , , Shahrokhabadi Khadijeh، Nejhad نويسنده Assistant Professor, Genetics, Dept. Biology, Islamic Azad University, Mashhad, Iran , , Maryam، Nazemi نويسنده M.Sc., Cell & Developmental Biology, Dept. Biology, Islamic Azad University, Mashhad, Iran ,
Abstract :
Studies on stem cells have created new possibilities for osseous defects therapies. This expremental study focused on the synergic effects of saffron extract and vitamin D3 on osteogenesis differentiation in bone marrow mesenchyme stem cells (BMSCs) of wistar rat. BMSCs were isolated from rat’s femur and treated with different doses of saffron extract, vitamin D3 and combination saffron extract and vitamin D3. Extract cytotoxicity was measured using MTT assay. The alkaline phosphatase (ALP) enzyme activity, Alizarin red staining and RT-PCR were performed to evaluate the differentiation progress. MTT assay indicated saffron extract, vitamin D3 and a combination used of those factor decreases cell viability. ALP activity showed that saffron extract, Vitamin D3 and application of two factors together increase ALP activity. Alizarin red staining showed vitamin D3, saffron and synergic used of them affects cell mineralization. RT-PCR result demonstrates in 14 days the group that treated with vitamin D3 and saffron Together express osteocalcin gene.
