Treatment Trial of Bovine Bacterial Mastitis in Khartoum State, Sudan

Mastitis is an inflammatory reaction of the udder tissue which is commonly caused by the microbial infection. Bovine mastitis is one of the devastating diseases causing huge loss to the dairy industry worldwide. The present study investigated the current status of clinical mastitis among dairy cattle in Khartoum state, Sudan. The prevalence of mastitis was assessed by measuring of milk pH and also based on the result of bacteriological evaluation of milk samples. A total of 100 isolates were recovered from 500 milk samples. The major pathogens isolated from the milk samples were : 31% Bacillus coagulans, 11% B. cereus, 9% B. subtilis, 9% B. licheniformis, 4% B. circulans, 2% B. lentus, 3% B. mycoides, 3% B. amyloliquefaciens, 2% B. megaterium, 16% Staphylococcus aureus, 8% Staphylococcus hyicus, 1% Corynebacterium spp. and 1% Klebsiella spp. Antibiogram studies were also conducted for the isolates by using twelve antibiotics including Chloramphenicol, Ciprofloxacin, Gentamycin, Tetracycline, Piperacillin/ Tazobactam, Pefloxacin, Amikacin, Ofloxacin, Co-Trimoxazole, Ceftizoxime, Cefotaxime and Ampicillin/ Sulbactam, which were used frequently in this area for the treatment of mastitis and Chloramphenicol and Ciprofloxacin were found to be more effective antibiotics among all the tested antibiotics. The effectiveness of different antibiotics in different isolates were as follows: Hundred percent of isolates were sensitive for Chloramphenicol and Ciprofloxacin, 91.6% for Gentamycin and Piperacillin/ Tazobactam, 83.3% for Pefloxacin and Tetracycline, 75.0% for Amikacin and Ofloxacin, 66.6% for Ceftizoxime, 33.3% for Co-Trimoxazole and Cefotaxime and 16.6% for Ampicillin/ Sulbactam.